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Prokazyme develops and sells enzymes for research, diagnostics and industrial testing. The Prokazyme-Matis alliance specialises in the exploitation of thermophilic microorganisms to develop novel enzyme products only available from us.

Prokazyme 开发并销售用于研究、诊断和工业检测的酶。Prokazyme 与 Matis 的联盟专注于利用嗜热微生物开发仅由我们提供的新型酶产品。

Key Product: A thermostable β-1,6-endoglucanase developed from environmental DNA

重点产品：从环境DNA开发的热稳定β-1,6-内切葡聚糖酶

产品货号：CEL136 / CEL136L
产品规格：100U 或 500U

A thermostable β-1,6-endoglucanase or pustulanase (E.C. 3.2.1.75) which catalyses the cleavage of β-1,6 bonds in pustulan and other beta-glucans containing 1,6 linked glucose units. The enzyme is now a component in a standard assay for beta-glucan from Baker’s yeast as documented in US Pharmacopeia Food and Chemicals Index 7th Edition, 3rd Supplement p. 1686-1689.

Available as 100 units or 500 units. Shipping within a week, shipping and handling charges additional to price listed below.

一种热稳定的β-1,6-内切葡聚糖酶（ pustulanase，EC 3.2.1.75），可催化切割pustulan和其他含有1,6连接葡萄糖单元的β-葡聚糖中的β-1,6键。该酶目前是美国药典《食品与化学品索引》第七版第三增补本第1686-1689页中记载的面包酵母β-葡聚糖标准检测方法的组成部分。

提供100单位或500单位包装。一周内发货，运费和手续费另计。

Enzyme Activity

酶活性

Cel136 Beta-glucanase (glucan endo-1,6-β-glucosidase) hydrolyses Beta-glucan polysaccharides with β-(1,6) linkages – see further below for substrate specificity

Cel136 β-葡聚糖酶（葡聚糖内切-1,6-β-葡萄糖苷酶）可水解具有β-(1,6)键的β-葡聚糖多糖——底物特异性详见下文

Activity Determination

活性测定

The standard assay for activity was done by incubating the enzyme at 80°C for 10 min. with 1% (w/v) pustulan as substrate in 0,1 M sodium phosphate buffer at pH 5.5. The reducing sugars released were detected by the dinitrosalisylic acid method using glucose as standard.

活性测定的标准方法是将酶在80°C下与1%（w/v）pustulan作为底物在0.1 M磷酸钠缓冲液（pH 5.5）中孵育10分钟。释放的还原糖通过二硝基水杨酸法检测，以葡萄糖为标准。

Unit definition

单位定义

One unit (U) of enzyme activity is the amount that leads to the release of 1 μmol reducing sugars per minute.

一个酶活性单位（U）是指每分钟释放1微摩尔还原糖所需的酶量。

Synonyms

同义词

glycoside hydrolase, pustulanase, glucan endo-1,6-β-glucosidase, 6-β-D-glucan glucanohydrolase, endo-1,6-β-glucanase; β-1→6)-β-D-glucanase; β-1,6-glucanase-pustulanase; β-1,6-glucan hydrolase; β-1,6-glucan 6-glucanohydrolase; 1,6-β-D-glucan glucanohydrolase

糖苷水解酶、pustulanase、葡聚糖内切-1,6-β-葡萄糖苷酶、6-β-D-葡聚糖葡聚糖水解酶、内切-1,6-β-葡聚糖酶；β-1→6)-β-D-葡聚糖酶；β-1,6-葡聚糖酶-pustulanase；β-1,6-葡聚糖水解酶；β-1,6-葡聚糖6-葡聚糖水解酶；1,6-β-D-葡聚糖葡聚糖水解酶

References

参考文献

  • Beta Glucan from Baker’s Yeast (Saccharomyces cerevisiae). Food Chemical Index, 7th Edition, Third supplement, p. 1686-1689. (2011) – includes assay for beta-glucan using the Cel136 product.
  • M. Danielson et al.: Enzymatic Method to Measure β-1,3-β-1,6-Glucan Content in Extracts and Formulated Products (GEM Assay), J. Agric. Food Chem. 58: 10305-10308 (2010).
  • 面包酵母（酿酒酵母）β-葡聚糖，《食品化学品索引》第七版第三增补本，第1686-1689页（2011年）——包含使用Cel136产品检测β-葡聚糖的方法。
  • M. Danielson等人：酶法测定提取物和配方产品中β-1,3-β-1,6-葡聚糖含量（GEM检测法），《农业与食品化学杂志》58卷：10305-10308（2010年）。